Comparison of complete rpoB gene sequence typing and multi-locus sequence typing for phylogenetic analysis of Staphylococcus aureus
نویسندگان
چکیده
منابع مشابه
[Multilocus sequence typing of methicillin-resistant Staphylococcus aureus].
OBJECTIVE To analyze multilocus sequence typing (MLST) of methicillin-resistant Staphylococcus aureus (MRSA) strains in 2000 and 2005, and get a primary knowledge of MLST Characterization of MRSA. METHODS Sequence analysis was conducted on seven allelic genes of 29 methicillin-resistant Staphylococcus aureus strains and 2 methicillin-sensitive Staphylococcus aureus (MSSA) strains and the alle...
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The genus Clostridium comprises a heterogeneous group of organisms for which the phylogeny and evolutionary relationships are poorly understood. The elucidation of these evolutionary relationships necessitates the use of experimental methods that can distinguish Clostridium lineages that are time and cost effective, and can be accurately and reproducibly employed in different laboratories. Mult...
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Multilocus sequence typing (MLST) is a sequence-based genotyping method based on polymorphisms (each variant is termed an allele) in seven housekeeping genes (loci) (arcC, aroE, glpF, gmk, pta, tpi, and yqiL) in Staphylococcus aureus providing unique allelic profiles known as sequence types (STs) (1). The data obtained by MLST are highly reproducible and are available in the S. aureus MLST data...
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A total of 258 bovine-associated Staphylococcus aureus isolates from the United States, Chile, and the United Kingdom, plus the reference isolate S. aureus Newbould 305 (NCIMB 702892), were analyzed by multilocus sequence typing (MLST). A collection of previously characterized United Kingdom isolates were also included in the analysis. The results demonstrated that MLST is suitable for the diff...
متن کاملMultilocus sequence typing of Staphylococcus aureus with DNA array technology.
A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventiona...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 2013
ISSN: 0022-1260,1349-8037
DOI: 10.2323/jgam.59.335